The superoxide scavenging activity of ethanolic extract of d. Carotene bleaching assay, superoxide anion radical scavenging assay and in vivo lipid peroxidation assay, assay of catalase cat activity, assay of reduced glutathione gsh concentration experimental models were used. Superoxide radical scavenging activity the superoxide anion scavenging activity was measured based on the described method 21. Reactive oxygen species ros, including free radicals such as superoxide anion radicalso. The crude aqueous extract of the plant contains the phenolics and flavonoids which are said to be the potent antioxidants 11. Hydroxyl radical scavenging assay, abts radical cation scavenging activity, antilipid peroxidation assay. The extract was examined for different reactive oxygen species ros scavenging activities including hydroxyl, superoxide, nitric oxide, hydrogen peroxide, peroxynitrite, singlet oxygen and. Free radicals, natural antioxidants, and their reaction. Radical scavenging and antioxidant activity of tannic acid. However, there has been no report on the antioxidant properties of this species. Superoxide radical scavenging and antibacterial activities. Invitro antioxidant and free radical scavenging activity of. This study was designed to investigate the superoxide radical scavenging and antibacterial properties of different. Nov, 2009 this study was designed to explore the antioxidant potential of hexane, chloroform, ethyl acetate, methanol, and water extract of bark and leaves of cassia siamea and cassia javanica by superoxide anion radical scavenging assay.
Superoxide radical scavenging properties of extracts and. The radical scavenging activity, antioxidant activity by carotene bleaching assay and, superoxide dismutase sod like ability decreased in the order of 70% methanol, 70% ethanol, cm, ea and, n. Molecular oxygen dioxygen is a diradical containing two unpaired electrons, and. In addition, aafb melanin exhibited stronger scavenging activities on 2,2diphenyllpicrylhydrazyl dpph radical, superoxide radical and hydroxyl radical with.
The free radical scavenging activity was found to be high in methanolic extract for dpph, hydroxyl, nitric oxide, superoxide, hydrogen peroxide h2o2 radical in. In vitro nitric oxide scavenging activity of methanol. Superoxide anion radical scavenging activity of bilberry extract fractions although the superoxide anion radical is a weak oxidant, it gives rise to the generation of more powerful and. Free radical scavenging activity of ethanolic extract of. Dpph free radical scavenging activity of the extracts of the. Superoxide anion and hydroxyl radical scavenging activities of. Apr 30, 2010 the ef showed strong 1,1diphenyl2picrylhydrazyl dpph radical scavenging activity, superoxide radical scavenging activity, and hydroxyl radical scavenging with a 50% inhibition concentration ic50 of 0.
Superoxide radical scavenging activity of the major. Antioxidant capacity and radical scavenging effect of polyphenol. Superoxide is produced as a byproduct of oxygen metabolism and, if not regulated, causes many types of cell damage. Lactobacillus fermentum exerted inhibitory effects on the generation of the superoxide radical, with 1286% scavenging activity at 10 6 cfu ml. Data obtained showed that the extract scavenged superoxide anion radicals ic 501602 gml in a dose dependant manner using. Pdf hydroxyl radical scavenging activity of flavonoids. The superoxide radical scavenging activity of the extract was assessed following the method of robak and gryglewski 34. Preparation of keratinglycine metal complexes and their. In vitro free radical scavenging activity of jatropha. Evaluation of free radical scavenging activity of an. Scheme 7 mechanism of dna damage induced by quercetin copper complex. Superoxide radical reduced nbt to a bluecolored formation that was measured at 560 nm. Oxygen radical scavenging activity of flavonoidschemistry, metabolism, cardioprotective effects, and dietary sources. Evaluation of antioxidant and free radical scavenging.
Introduction the superoxide radical is ubiquitous in aerobic cells cerutti, 1994. Each extract was selected to study the fr scavenging activity by superoxide scavenging assay and 2, 2diphenyl1 picrylhydrazyl hydrate dpph radical scavenging assay method. Anacardiaceae on superoxide radical scavenging and xanthine oxidase activity ferda candan faculty of science and art, department of chemistry, university of cumhuriyet, 58140 sivas, turkey received august 2002 rhus coriaria l. The super oxide anion radical scavenging activity of a. It was found that aqueous extract contained carbohydrates, glycosides amino acids flavonoids, tannins, alkaloids and steroids. The present study is to investigate the antioxidant activity of ethanol extract of flowers of spathodea campanulata through nitric oxide radical, superoxide radical scavenging assay and phosphomolybdonum method. Chemical composition and radical scavenging activity of. Free radical scavenging and antioxidant activities of.
Pdf hydrogen peroxide radical scavenging and total. Antioxidant and free radical scavenging activity of spondias. Pdf potential superoxide anion radical scavenging activity. The objective of the present study was to evaluate the antioxidant potential and free radical scavenging activity of a 70% methanol extract of s. Free radical scavenging activities of watersoluble extracts from some natural sources, health foods, and antioxidant substances were measured using the jes. The main methods comprise superoxide radicals scavenging o 2. In vitro antioxidant activities, free radical scavenging.
We found that the srsa levels of the polyphenols were closely related to their chemical structures. Activity of superoxide dismutase enzyme was evaluated based on the ability of sod to inhibit the oxidation. Free radical scavenging activity of fruiting body extracts of. The antioxidant activity of the memq was evaluated by the. Methods used to evaluate the free radical scavenging. Assay of nitric oxide scavenging activity nitric oxide scavenging activity can be estimated by the use of griess iliosvoy reaction garrat, 1964. The different extracts showed significant inhibition of superoxide radicals in a dosedependant manner. This becomes of major concern in the case of pure components or complex samples that have been isolated in low extent. It was found that the scavenging activity of fkgly was best and ec 50 and degree of simulation could, respectively, reach. Free radical scavenging activity of lactobacillus fermentum. Superoxide anion radical scavenging activity biology essay. A 70% methanol extract of spondias pinnata stem bark was studied in vitro for total antioxidant activity, for scavenging of hydroxyl radicals, superoxide anions, nitric oxide, hydrogen peroxide, peroxynitrite, singlet oxygen and hypochlorous acid, and for iron chelating capacity, reducing power, and phenolic and flavonoid contents. Simplified methods for microtiter based analysis of in vitro.
The contribution of the pyrogallol moiety to the superoxide. Superoxide anion radical scavenging activity superoxide radical o2 was generated from the photoreduction of riboflavin and was deducted by nitro blue tetrazolium dye nbt reduction method. Free radicals and other reactive oxygen species are recognized as agents involved in the pathogenesis of many ailments. The reactive oxygen ion superoxide is particularly important as the product of the oneelectron reduction of dioxygen o 2, which occurs widely in nature. Free radical scavenging activity of the methanol extract was tested in three in vitro models, viz. The effect of polyphenols among different varieties of plums on superoxide radical scavenging activity srsa was studied by an enzymatic method and their ic 50 values were determined. Superoxide radicals were generated in a pmsnadh system by oxidation of nadh and assayed through reduction of nbt. Free radical scavenging activity of lupeol isolated from the. In vitro antioxidant and free radical scavenging activity 39 roots are the main portions of the whole plant as they possess wide number of the therapeutic agents. The % hydroxyl radical scavenging activity of test sample was determined accordingly in comparison with negative control. There are also inconsistencies regarding the relative o 2 2 scavenging activity of. In addition, aafb melanin exhibited stronger scavenging activities on 2,2 diphenyllpicrylhydrazyl dpph radical, superoxide radical and hydroxyl radical with. In order to investigate the antioxidant activity, different complementary test systems, namely, 2,2diphenyl1picrylhydrazil dpph free radical scavenging, total phenolic, total tannin and. Superoxide free radical scavenging activity the naringin scavenging effect on superoxide free radical activity was measured as by the procedure developed by hyland et al.
Superoxide radical scavenging activity and reducing capacity. Comparative study of abts radical scavenging activity and. The test was carried out using perkin elmer victor 4x micro plate reader performed in a 96 well plate using a total volume of 200 l methanol containing 0. The antioxidant activity of the tannic acid, bha, bht. Free radical scavenging, angiotensin iconverting enzyme ace.
Although only mildly reactive toward biological molecules, the superoxide radical may be. In vitro antioxidant and free radical scavenging activity of. Hydroxyl and superoxide anion radical scavenging activities of. Antioxidant and free radical scavenging activity of. Our finding further provides evidence that mallotus fruit extract is a potential natural source of antioxidants which have a protective role on human erythrocytes exhibiting minimum hemolytic activity and. Free radical scavenging activities of watersoluble extracts from some natural sources. The ic50 value table 1 of the test sample was centage inhibition of deoxyribose degradation. Scavenging definition of scavenging by the free dictionary.
Total phenol and total flavonoid content in extracts, which reason antioxidant and free radical scavenging activity, was. The antioxidant activity of the aqueous ethanolic extract of doum leaves, hyphaene thebaica l. Anthocynidine, a class of flavonoids are potential antioxidants and their effectiveness in the inhibition of the lipid oxidation is related to their metal ionchelating activity scheme 8. The antioxidant activity of the aerial part extract of m. Superoxide, hydroxyl radical, lipid peroxidation, nitric oxide assay showed a comparable scavenging activity compared to its standard. The superiority of pyrogallol over catechol in the flavonoidal nucleus is apparent from the much higher o 2. Hydroxy radical and dpph scavenging activity of crude protein. In this experiment, the superoxide radicals were generated in 3 ml of sodium phosphate buffer.
Different parts and different plants showed different levels of super oxide anion radical scavenging activity and reducing capacity. Pdf nitric oxide and superoxide radical scavenging activity. After adding different concentrations of eps7630 to the mixture, the absorption was measured at 560 nm, indicating the consumption of superoxide anion radicals in the mixture. The compound sodium nitroprusside is known to decompose in aqueous solution at physiological ph 7. A superoxide is a compound that contains the superoxide ion, which has the chemical formula o.
Dec 09, 2008 the objective of the present study was to evaluate the antioxidant potential and free radical scavenging activity of a 70% methanol extract of s. Estimation of peroxidase activity peroxidase catalyses the oxidation of a variety of electron donors with the help of h 2 o 2 thus scavenging the. Mentha spicata is a wellknown spice that has a variety of biological properties and is abundantly available throughout the world. Dpph test free radicalscavenging activity of each silymarin components was carried out using the dpph scavenging method 21. Measurement of superoxide anion scavenging activity was performed based on the method described by winterbourne et al 186. Superoxide radical scavenging activity by alkaline.
Antioxidant capacity and radical scavenging effect of. In this work are described the methods used for this purpose in both substrates as well as in specific cases of their application. On a dry weight basis, eggplant, and red, yellow and green bell pepper extracts showed potent superoxide anion radical scavenging activities sodlike activities. The reaction mixture contains various concentrations of naringin100500. Anacardiaceae is a wellknown spice in the middleeast and grown in the central. The antioxidant properties of carthamustinctorius ethanol extract was evaluated using superoxide anion radical scavenging activities.
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